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Purpose: To evaluate the techniques of rapid and accurate diagnosis of chlamydialconjunctivitis.Methods: Total 100 conjunctivitis patients (200 eyes) were studied. Twenty-two of100 cases were diagnosed as chlamydial conjunctivitis. The infected epithelia werescraped from tarsal conjunctiva of both eyes and stained separately with Giemsa (100cases) and immunofluorescence (anti-chlamydial antigen monoclonal antibody, 100cases).Result: In immunofluorescent staining, 38 cases were seen positive staining and 62were negative. In Giemsa staining, 29 were positive, and 71 were negative. In 22 caseswith clinical diagnosis of chlamydial conjunctivitis, 13 cases were confirmed, and 9were excluded by immunofluorescent staining. Technically, immunofluorescent andGiemsa stain takes 45 and 40 minutes, respectively.Conclusion: Comparing to Giemsa stain, 38 of 100 scraping specimens were positive(38% ) by immunofluorescent staining, 29 of 100 per cent were positive by Giemsastaining. Giemsa staining takes 5 minutes less
Objective: To evaluate the techniques of rapid and accurate diagnosis of chlamydialconjunctivitis. Methods: Total 100 conjunctivitis patients (200 eyes) were studied. Twenty-two of 100 cases were diagnosed as chlamydial conjunctivitis. The infected epithelia werescraped from tarsal conjunctiva of both eyes and stained In Giemsa staining, 29 were positive and 71 were negative. In 22: negative with Giemsa (100cases) and immunofluorescence (anti-chlamydial antigen monoclonal antibody, 100cases) Caseswith clinical diagnosis of chlamydial conjunctivitis, 13 cases were confirmed, and 9were excluded by immunofluorescent staining. Technically, immunofluorescent and Giemsa stains took 45 and 40 minutes, respectively. Conlusion: Comparing to Giemsa stain, 38 of 100 scraping specimens were positive (38%) by immunofluorescent staining, 29 of 100 per cent were positive by Giemsastaining. Giemsa staining ta kes 5 minutes less