宫颈病变患者人乳头状瘤病毒16的生物状态和晚期蛋白l1表达

• 作 者: 陈宝焕 时艳梅 严倩 林爽
• 机 构: 山东省烟台市烟台山医院妇科,264001,山东省烟台市烟台山医院妇科,264001,山东省烟台市烟台山医院妇科,264001,山东省烟台市烟台山医院妇科,264001
• 发表日期: 2011年4期
• 关键词: 乳头状瘤病毒感染 宫颈病变 生物状态 L1蛋白

目的 探讨人乳头状瘤病毒(hpv)16的存在状态及晚期蛋白l1表达与宫颈病变程度的关系.方法 收集61例宫颈病变患者为研究对象,按病理学诊断结果分为慢性宫颈炎组(27例),cin Ⅰ、cinⅡ组(10例),原位癌组(8例),早期浸润癌组(7例)和中晚期癌组(9例).采用pcr方法检测hpv分型及生物状态,采用免疫印迹法(western blot)测定晚期蛋白l1表达.组间比较采用kruskal-wallis检验,hpv16生物状态、l1蛋白表达水平与病变程度的关系采用pearson相关分析.结果 61例宫颈病变患者检出hpv16型53例,占86.9%,其中25例慢性宫颈炎患者均为游离型;cin Ⅰ、cinⅡ组6例游离型,2例整合型;原位癌组1例游离型,3例混合型和3例整合型;早期浸润癌组2例混合型,4例整合型;中晚期癌组1例混合型,6例整合型.hpv16整合型与宫颈病变程度呈正相关(r=0.705,p＜0.01).随着宫颈病变损伤程度加重,l1蛋白表达逐渐减弱,与宫颈病变程度呈负相关(r=-0.755,p＜0.01).结论 hpv16整合型及l1蛋白表达下降预示宫颈病变程度加重.; abstract：; objective to evaluate the relationship of biological status of hpv 16 and expression of l1 protein with the degree of cervical tumorigenesis.methods sixty-one patients with cervical lessions were enrolled and divided into five groups according to pathology of cervical lesions,including chronic cervicitis(n = 27),cervical intraepithelial neoplasia(cin) Ⅰ-Ⅱ(n = 10),carcinoma in situ(n = 8),early-stage(n =7)and mid/late-stage(n = 9)of cervical carcinoma.hpv type and its biological status were detected by pcr amplification,and l1 protein in hpv 16 positive tissues was detected by western blot.kruskal-wallis was used to compare between the groups,and pearson correlation analysis was used to evaluate the relationship of hpv biological status and l1 protain expression with cervical lesions.results fifty-three out of 61 patients with cervical lesions were detected with hpv positive(86.9%).all 25 patients with chronic cervicitis were in hpv free mode; in cin Ⅰ-Ⅱ group,6 patients were in free and 2 in integral mode; in carcinoma in situ group,1 patient in free,3 in mixed and 3 in integral mode; in the early-stage cervical cancer group,2 patients in mixed and 4 in integral mode; in mid/late-stage cancer group,1 patient in mixed and 6 in integral mode.a strong positive correlation was found between the hpv in integral mode and the severity of cervical lesions(r = 0.705,p ＜0.01).the expression of l1 protein was negatively correlated with the aggravation of cervical lesions in hpv 16-positive patients(r = -0.755,p ＜0.01).conclusion the integral mode of hpv16 and low expression of l1 protein may have predictive value for the severity of cervical lesions.