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目的观察首乌丹参方(GTSMP)对大鼠心肌缺血再灌注损伤(I/R)保护作用。方法将实验大鼠分为假手术组、模型组、首乌丹参方高剂量组(9g生药/kg)、首乌丹参方中剂量组(4.5g生药/kg)、首乌丹参方低剂量组(2.25g生药/kg)、阳性对照药复方丹参滴丸组(4.5g生药/kg)、工具对照药消心痛组(1.67mg/kg),共7组。采用结扎大鼠冠状动脉前降支30min/开放120min建立心肌I/R模型。通过RT-PCR分子生物学方法,观察蛋白激酶C(PKC) mRNA和iNOS mRNA的表达情况以及首乌丹参方预处理对其的影响。结果与假手术组相比,模型组和首乌丹参方各组PKC mRNA表达均有所下降;与模型组相比,首乌丹参方中剂量组、复方丹参滴丸组和消心痛组表达上调,均表现出显著性差异,首乌丹参方高剂量组及低剂量组也能促进PKC mRNA的表达,但没有显著性差异;假手术组心肌iNOS mRNA弱表达,模型组呈现高表达,首乌丹参方可下调iNOS mRNA基因表达。结论首乌丹参方预处理可促进I/R的大鼠心肌的PKC表达,下调I/R大鼠心肌iNOS mRNA的表达;其通过激动PKC,使心肌组织iNOS mRNA弱表达,可能是首乌丹参方对缺血再灌注损伤的心肌的保护效应机制之一。
Objective To observe the protective effect of Shouwu Danshen prescription (GTSMP) on myocardial ischemia-reperfusion injury (I/R) in rats. Methods Rats were divided into sham operation group, model group, Shouwu Danshenfang high dose group (9g crude drug/kg), Shouwu Danshenfang middle dose group (4.5g crude drug/kg), Shouwu Danshenfang low dose group. (2.25g crude drug/kg), positive control compound compound Danshen Dripping Pills (4.5g crude drug/kg), and instrumental control drugs Xiaodutong (1.67mg/kg), a total of 7 groups. Myocardial I/R model was established by ligation of anterior descending coronary artery in rats for 30 min/120 min. The expression of protein kinase C (PKC) mRNA and iNOS mRNA was observed by RT-PCR molecular biology method, and the effect of Shouwu Danshen Pretreatment on it was observed. Results Compared with the sham-operated group, the expression of PKC mRNA in the model group and Shouwu Danshen prescription group decreased. Compared with the model group, the expression of Shouwu Danshen prescription middle dose group, compound Danshen Dripping Pills group, and Xiaoxintong group were up-regulated. , All showed significant differences. Shouwu Danshenfang high dose group and low dose group also can promote the expression of PKC mRNA, but there was no significant difference; sham operation group myocardial iNOS mRNA weak expression, the model group showed high expression, Shouwu Salvia miltiorrhiza can down-regulate iNOS mRNA expression. Conclusion Shouwu salvia miltiorrhiza preconditioning can promote the expression of PKC in I/R rat myocardium and down-regulate the expression of iNOS mRNA in I/R rat heart. By aggravating PKC, the expression of iNOS mRNA in myocardium tissue is weak, which may be Shouwu Salvia miltiorrhiza. One of the protective effects of Fang on myocardial ischemia-reperfusion injury.