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目的 :研究六味地黄汤及其药物血清对血管内皮细胞 (VEC)损伤模型的保护作用。方法 :本实验利用大肠杆菌内毒素 (LPS)致VEC凋亡模型 ,并通过MTT、细胞周期和凋亡细胞的检测 ,观察了药物血清对VEC的保护作用 ,并通过MDA、SOD等指标探讨其作用机理。结果 :六味地黄汤药物血清可减轻LPS对VEC的损伤 ,抑制VEC凋亡发生 ,促进VEC增殖 ;并可通过增加VEC分泌SOD ,清除MDA ,而达到其减轻LPS对内皮细胞损伤的目的。结论 :六味地黄汤具有保护VEC损伤模型的作用 ,这种作用将有助于血栓性疾病的防治。
Objective : To study the protective effect of Liuweidihuang Decoction and its drug serum on vascular endothelial cell (VEC) injury model. METHODS: In this experiment, the VEC apoptosis model induced by E. coli endotoxin (LPS) was used to detect the protective effect of drug serum on VEC through MTT assay, cell cycle, and apoptotic cells. Mechanism of action. Results : The serum of Liuwei Dihuang Decoction could reduce the damage of VEC to VEC, inhibit the apoptosis of VEC, promote the proliferation of VEC, and reduce the damage of LPS to endothelial cells by increasing the secretion of SOD and the removal of MDA by VEC. Conclusion : Liuwei Dihuang Decoction has the effect of protecting VEC injury model, which will help the prevention and treatment of thrombotic diseases.