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目的:探讨恶性阻塞性黄疸患者术后给予利胆扶正颗粒治疗对外周血CD4+CD25+调节性T细胞(regulatory T-Lymphocytes,Treg)的调节作用及意义。方法:30例恶性阻塞性黄疸一般治疗组,30例恶性阻塞黄疸中药治疗组,30例良性阻塞性黄疸组和30例健康对照组。在胆道梗阻解除后采用流式细胞术对外周血Treg数量进行检测,观察Treg数量与CD4+和CD8+T细胞的关系。结果:(1)与健康对照者比较,阻塞性黄疸患者(包括良性、恶性患者)Treg水平显著上升(均P<0.05);恶性阻塞性黄疸患者Treg水平较良性为高(P<0.05);(2)恶性阻塞性黄疸中药治疗组与一般治疗组比较Treg水平有所下降(P<0.05),CD4+和CD8+T细胞水平有所上升(P<0.05)。结论:恶性阻塞性黄疸患者外周血Treg数量明显升高,存在免疫调节紊乱。利胆扶正颗粒对机体细胞免疫有重要的辅助作用,具有一定的免疫调节作用。
Objective: To investigate the regulatory effect of Liguan Fuzheng granule on the expression of CD4 + CD25 + regulatory T-Lymphocytes (Treg) in peripheral blood of patients with malignant obstructive jaundice. Methods: Thirty cases of general obstructive jaundice, 30 cases of traditional Chinese medicine treatment of malignant obstructive jaundice, 30 cases of benign obstructive jaundice and 30 healthy controls were included. The number of Tregs in peripheral blood was detected by flow cytometry after the biliary obstruction was relieved, and the relationship between the number of Tregs and CD4 + and CD8 + T cells was observed. Results: (1) Treg levels in obstructive jaundice patients (including benign and malignant patients) were significantly higher than those in healthy controls (all P <0.05); Treg levels were higher in patients with malignant obstructive jaundice than in benign patients (P <0.05); (2) The level of Treg in Chinese medicine treatment group was lower than that in general treatment group (P <0.05), and the level of CD4 + and CD8 + T cells increased (P <0.05). Conclusion: The number of Tregs in peripheral blood of patients with malignant obstructive jaundice is obviously increased, and there is disorder of immune regulation. Li Dan Fuzheng particles on the body cell immune has an important supporting role, with a certain degree of immune regulation.