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目的 通过研究HIV - 1Vpr基因在宫颈癌细胞HeLa中诱导细胞周期G2停滞、细胞致死效应及其核定位功能 ,探讨将Vpr用于肿瘤治疗的可能性。方法 用脂质体转染的方法 ,将携带Vpr或其突变体VprX基因的质粒转入HeLa细胞内 ,用流式细胞仪分析Vpr诱导的G2停滞和细胞致死性 ;用荧光显微镜观察GFP -Vpr融合蛋白的荧光确定其核定位功能。结果 Vpr具有核定位功能。Vpr和VprX蛋白都具有细胞致死性 ,但是VprX蛋白不能使细胞停滞到G2期。结论 首次报道了Vpr的G2期停滞和细胞致死功能在哺乳动物细胞中是互相独立的。Vpr在肿瘤治疗中有很大的潜力
Objective To study the possibility of using Vpr for tumor therapy by studying the effect of HIV-1 Vpr gene on cell cycle G2 arrest, cell lethality and its nuclear localization in cervical cancer HeLa. Methods Plasmids carrying Vpr or its mutant VprX gene were transfected into HeLa cells by liposome transfection. Vpr-induced G2 arrest and cell lethality were analyzed by flow cytometry; GFP-Vpr was observed by fluorescence microscopy. The fluorescence of the fusion protein determines its nuclear localization function. Results Vpr has a nuclear location function. Both Vpr and VprX proteins have cell lethality, but VprX protein does not arrest the cells to G2. Conclusion It was reported for the first time that the G2 arrest and cell lethal function of Vpr are independent of each other in mammalian cells. Vpr has great potential in cancer treatment