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[目的]观测杞参膏对干眼症SD大鼠血清Mig、IP-10影响。[方法]使用随机平行对照方法,30只SD大鼠喂养7d,称重后按照随机数字法分为3组,空白对照组、模型对照组、杞参膏组,10只/组。适应喂养7d后,暴露和皮下注射东莨菪碱法复制干眼症大鼠模型,连续28d。灌胃干预,模型对照组:生理盐水,1次/d;杞参膏组:杞参膏汤剂,1次/d;均连续56d。观测角膜病理、血清Mig、IP-10含量。[结果]空白对照组大鼠角膜未见异常。模型对照组大鼠角膜上皮层细胞增多,层数增加,局部扁平细胞偶见不规则的脱失;基质层可见增厚表现,偶见水肿,大量炎性细胞浸润。杞参膏组大鼠角膜各层尚为完整,上皮层光滑度尚可,未见扁平细胞脱失现象。与空白对照组比较,模型对照组大鼠血清Mig、IP-10含量显著升高(P<0.01);与模型对照组比较,杞参膏组大鼠血清Mig、IP-10含量显著降低(P<0.01,P<0.05)。[结论]杞参膏可减轻干眼症模型大鼠角膜病理损伤,降低干眼症模型大鼠血清Mig、IP-10含量,可能是杞参膏抗炎作用的机制之一。
[Objective] To observe the effect of Qi-ginseng extract on serum Mig and IP-10 in SD rats with dry eye. [Methods] Thirty SD rats were fed for 7 days with randomized parallel control. After weighing, they were divided into three groups according to random number method: blank control group, model control group, Qi ginseng cream group and 10 rats / group. After 7 days of feeding, rats were exposed to scopolamine for subcutaneous injection for 28 days. Gavage intervention, the model control group: saline, 1 / d; Qi ginseng paste group: Qi ginseng paste, 1 / d; were continuous 56d. Corneal pathology, serum Mig, IP-10 content were observed. [Results] The corneas of blank control group showed no abnormality. The model control group rat corneal epithelial cells increased, the number of layers increased, the local flat cells occasionally irregular loss; stroma showed thickening performance, occasionally edema, a large number of inflammatory cell infiltration. Qi ginseng paste group rat corneal layer is still intact, the smoothness of the epithelium is acceptable, no flat cell loss phenomenon. Compared with the control group, the contents of Mig and IP-10 in the model control group were significantly increased (P <0.01). Compared with the model control group, the contents of Mig and IP-10 in the QiGan cream group were significantly decreased (P <0.01, P <0.05). [Conclusion] Qi ginseng cream can alleviate the corneal pathological injury of dry eye mouse model and reduce the content of Mig and IP-10 in the serum of dry eye mouse model, which may be one of the mechanisms of Qi ginseng cream anti-inflammatory effect.