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运用nm2 3基因的寡核苷酶探针 ,通过Northern印迹转移的方法 ,检测了 9例肾癌及 19例膀胱癌手术切除标本的nm2 3基因的表达产物mRNA的水平。结果显示 :⑴肾癌标本的nm2 3mRNA的水平增高(10 14± 3 4 6 ) ,与正常对照组相比 (3 4 6± 1 94 )差异有非常显著性 (t=3 172 ,P <0 0 1)。⑵膀胱癌标本nm2 3mRNA水平增高 (7 79± 3 4 1,n =19) ,与正常对照组相比 (3 0 8± 1 99,n =6 )差异有非常显著性 (t =2 90 4 ,P <0 0 1) ;膀胱肿瘤中不同的分期之间和肿瘤细胞的分级之间差异无显著性 (P >0 0 5) ,显示肾癌和膀胱癌中nm2 3基因mRNA表达量增高 ,且表达量与膀胱癌的分期和分级无关。
Using nm2 3 gene oligonucleotide probe, the expression of mRNA of nm23 gene in 9 cases of renal cell carcinoma and 19 cases of bladder cancer resected specimens was detected by Northern blotting. The results showed that: (1) The level of nm23 mRNA in renal cell carcinoma increased (10 14 ± 34 6) compared with the control group (34 6 ± 1 94) (t = 3 172, P <0 0 1). (2) The level of nm23mRNA in bladder cancer was significantly higher than that in the control group (789 ± 341, n = 19) (t = 2 904 , P <0.01). There was no significant difference between the different staging of bladder tumor and the grade of tumor cells (P> 0.05), which showed that the mRNA expression of nm23 gene in renal cell carcinoma and bladder cancer increased, And the expression of bladder cancer stage and grade has nothing to do.