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果蝇的早期心脏发育模式与脊椎动物的早期心脏发育模式具有惊人的相似性 ,研究果蝇心脏发育基因有助于揭示人体心脏发育机理和先天性心脏病发生机制。为了克隆和鉴定控制心脏发育的新基因 ,在化学诱变建立果蝇平衡致死系的基础上 ,用果蝇心脏组织特异抗体MabNo.3,对 310个平衡致死系进行免疫化学方法筛选 ,观察到有 6 3个致死系表现出心脏突变表型。分别将这些有心脏表型突变的品系与果蝇第 2和第 3染色体缺失系杂交 ,测定了 14个品系的遗传学位点 ,其中 7个品系在遗传学位点上有别于已报道的心脏发育控制基因。并查寻和讨论了各突变品系的可能侯选基因
Drosophila early pattern of heart development and vertebrates early pattern of cardiac development with a striking similarity to study Drosophila cardiac development genes help to reveal the mechanism of human heart development and congenital heart disease mechanism. In order to clone and identify new genes controlling cardiac development, based on the chemical mutagenesis to establish a balanced lethal strain of Drosophila, 310 Balancing lethal strains were screened by immunochemistry using MabNo.3, a heart-specific antibody against Drosophila. Sixty-three lethal strains showed a phenotype of cardiac mutations. The lines with these cardiac phenotypic mutations were crossed with chromosomes 2 and 3, respectively, of the flies, and the genetic loci of 14 lines were determined, of which 7 were genetically different from the reported heart development Control genes. And searched and discussed the possible candidate genes of each mutant line