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目的:探讨儿童哮喘急性发作期外周血单核细胞NF-κB活性与血清IL-4、IgE的水平变化及临床意义。方法:本研究设正常对照组52例,哮喘急性发作组84例,应用ELISA法检测血清IL-4、IgE水平,并提取外周血单核细胞用电泳迁移率分析法(EMSA)测定NF-κB活性。结果:在儿童哮喘急性发作组,外周血单核细胞NF-κB活性(0.793±0.062)明显高于正常对照组(0.643±0.071)(P<0.01);儿童哮喘急性发作组血清IL-4(108.2±41.7)ng/L显著高于正常对照组(38.2±15.3)ng/L;儿童哮喘急性发作组血清IgE(165.3±50.7)KU/L也显著高于正常对照组(45.8±14.2)KU/L;儿童哮喘急性发作组NF-κB活性与血清IL-4、IgE水平呈正相关(r=0.624、0.791,P<0.01)。结论:儿童哮喘急性发作期外周血单核细胞NF-κB活性明显增加,可能参与了放大炎症信号及调节炎症持续过程,抑制NF-κB活性可能下调IL-4、IgE的表达,能在较高的水平上调控产生炎性因子的总体环节,更有效地控制哮喘。
Objective: To investigate the changes of NF-κB activity in peripheral blood mononuclear cells and serum levels of IL-4 and IgE in children with acute asthma and their clinical significance. Methods: In this study, 52 cases of normal control group and 84 cases of acute exacerbation of asthma group were collected. Serum IL-4 and IgE levels were measured by ELISA. Peripheral blood mononuclear cells were extracted for determination of NF-κB by electrophoretic mobility shift assay (EMSA) active. Results: In acute exacerbation of childhood asthma, the activity of NF-κB in peripheral blood mononuclear cells (0.793 ± 0.062) was significantly higher than that of normal control group (0.643 ± 0.071) (P <0.01) 108.2 ± 41.7 ng / L was significantly higher than that of the normal control group (38.2 ± 15.3) ng / L. The serum IgE (165.3 ± 50.7) KU / L in acute asthma children group was also significantly higher than that of the normal control group (45.8 ± 14.2) KU / L. There was a positive correlation between NF-κB activity and IL-4 and IgE levels in children with acute asthma (r = 0.624,0.791, P <0.01). CONCLUSION: NF-κB activity in peripheral blood mononuclear cells is significantly increased in children with acute asthma, which may be involved in amplifying inflammatory signals and regulating the process of inflammation. Inhibition of NF-κB activity may down-regulate the expression of IL-4 and IgE, The level of regulation of inflammatory cytokines produce the overall link, more effective in the control of asthma.