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目的观察４１℃６０ｍｉｎ温热作用后人胃癌细胞ＢＧＣ－８２３热耐受性和ＨＳＰ７０表达的变化。方法４１℃６０ｍｉｎ温热作用后不同时间，测定细胞４３℃６０ｍｉｎ温热再作用的克隆形成率，并进行细胞ＨＳＰ７０免疫组化分析。结果①４１℃诱导后即时细胞已形成较高的热耐受性，４ｈ有所降低，８ｈ达到高峰（Ｐ＜０．０１），２４ｈ恢复近原有水平；②ＢＧＣ－８２３细胞常温下表达少量ＨＳＰ７０，并且主要分布于胞浆内；４１℃诱导后即时ＨＳＰ７０的表达量并未增加，但核内染色加深（Ｐ＜０．０１）；诱导后４ｈ开始增加（Ｐ＜０．０１），８～１６ｈ达到高峰，并主要在核内（Ｐ＜０．０１）；２４ｈ衰减至近原有水平（Ｐ＞０．０５）。结论４１℃温热诱导后ＢＧＣ－８２３细胞形成的热耐受性包括两部分：一部分为即时、非ＨＳＰ７０依赖的，另一部分为ＨＳＰ７０依赖的热耐受性。
Objective To observe the changes of heat tolerance and HSP70 expression in human gastric cancer cells BGC-823 after warming at 41°C for 60 min. Methods The rate of colony formation in cells at 43°C for 60 min after warming was measured at 41°C for 60 min. The cloning rate was measured and the HSP70 immunohistochemical analysis was performed. RESULTS: Immediately after 141°C induction, the cells had a high thermal tolerance, which decreased at 4 h, peaked at 8 h (P<0.01), and recovered to the original levels at 24 h; 2 BGC-823 cells expressed a small amount of HSP70 at room temperature. And mainly distributed in the cytoplasm; immediately after induction at 41 °C, the expression of HSP70 did not increase, but the nuclear staining increased (P<0.01); it increased at 4 hours after induction (P<0.01), 8 to 16 hours. Peaks were reached and mainly in the nucleus (P<0.01); 24h decayed to near original levels (P>0.05). Conclusion The heat tolerance of BGC-823 cells induced by warming at 41°C includes two parts: one is immediate, non-HSP70 dependent, and the other is HSP70-dependent thermal tolerance.