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应用10%脱脂乳、35%甘油肉汤和鉴定卡分别在-80℃保存海洋细菌,在保存3个月、6个月、1 a和2 a后分别复苏菌种,进行菌种的生化鉴定和药敏测评。发现10%脱脂乳和35%的甘油肉汤对各种菌种的保存效果都很好,到2 a时复苏率和鉴定率都为100%;鉴定卡对肠杆菌、非发酵和肠球菌保存到2 a时的复苏率和鉴定率为100%,但是对弧菌的复苏率则在保存3个月和6个月时为100%,到1 a时降低为80%,到2 a时仅为30%。认为10%脱脂乳和35%甘油肉汤在海洋细菌保存中效果较好,可以长期保存;鉴定卡短期保存细菌是可行的。
The marine bacteria were stored at -80 ℃ with 10% skim milk, 35% glycerol broth and identification card respectively. The strains were rescued after 3 months, 6 months, 1 a and 2 years respectively, And drug sensitivity evaluation. Found 10% skim milk and 35% of the glycerol broth preservation of various species are very good, to 2 a recovery rate and identification rate of 100%; identification card for Enterobacteriaceae, non-fermented and enterococci preservation The recovery rate and identification rate were 100% at 2 years, but the recovery rate to Vibrio was 100% at 3 months and 6 months and 80% at 1 year, and only at 2 years Is 30%. It is considered that 10% skim milk and 35% glycerol broth are effective in the preservation of marine bacteria and can be stored for a long time. It is feasible to use identification cards for short-term preservation of bacteria.