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成年大鼠50只,分肝部分切除组、假手术组和正常组。3组动物于术后1、2、3、7和14 d分批取材,进行以下组织学和组织化学染色:HE、Mallory、PAS、RNA、SH基和SS基、磷钼酸苏木精(PTAH)法、SDH、AcP、NE、G 6 P_1se、5-Nase,计算颗粒曲管(GCT)中三种细胞——富颗粒细胞,少颗粒细胞和无颗粒细胞所占百分比,以判断GCT细胞分泌活动的变化。结果表明,假手术动物颌下腺GCT细胞呈现一定程度的变化,以术后第1~2d较明显,GCT细胞内颗粒数量减少;PAS阳性、SS基反应阳性以及PTAH阳性的
50 adult rats were divided into partial liver resection group, sham operation group and normal group. Three groups of animals were drawn in batches on 1, 2, 3, 7 and 14 days after operation for the following histological and histochemical staining: HE, Mallory, PAS, RNA, SH and SS, phosphomolybdic acid hematoxylin PTAH), SDH, AcP, NE, G 6 P 1se, 5-Nase were used to calculate the percentages of the three cell-rich granulocytes, few granulocytes and granulosa cells in GCT to determine whether GCT cells Changes in secretory activity. The results showed that the number of GCT cells in the submandibular glands of the sham-operated animals changed to some extent, and the number of GCT cells decreased obviously on the first day to the second day after surgery. The number of GCT cells in PAS positive, SS-positive and PTAH-positive