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目的:观察参芎注射液对家兔离体心脏缺血再灌注损伤模型的冠脉流量、心肌组织匀浆中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响,以探讨其对离体心肌缺血再灌注损伤的保护作用及可能机制。方法:采用Langendor-ff离体心脏恒压灌流方法,结扎左冠状动脉前降支30min再复灌法复制离体心肌缺血再灌注损伤模型,测定冠脉流量,制备心肌组织匀浆检测SOD的活性和MDA的含量。结果:与假手术组比较,模型组各时间点冠脉流量均减低,组织中SOD活性减弱,MDA含量增加;与模型组比较,参芎组各时间点冠脉流量升高,组织中SOD活性增强,MDA含量减少。结论:参芎注射液能够保护缺血再灌注损伤的心肌,其可能机制与增加冠脉流量、增强SOD活性、降低MDA含量有关。
Objective: To observe the effect of Shenxiong injection on coronary flow, activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) in myocardial ischemia-reperfusion injury in isolated rabbit hearts Its protective effect on isolated myocardial ischemia-reperfusion injury and its possible mechanism. Methods: Langendor-ff isolated cardiac perfusion method was used to ligate the left anterior descending coronary artery for 30 minutes and reperfusion to duplicate the model of myocardial ischemia-reperfusion injury. The coronary flow rate was measured and the myocardial tissue homogenate was prepared for the detection of SOD Activity and MDA content. Results: Compared with the sham-operation group, the coronary flow decreased and the activity of SOD decreased in the model group at each time point, and the content of MDA increased. Compared with the model group, the coronary flow increased and the SOD activity Enhanced MDA content decreased. Conclusion: Shenxiong injection can protect myocardium from ischemia-reperfusion injury. Its mechanism may be related to increasing coronary flow, increasing SOD activity and decreasing MDA content.