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目的检测非小细胞肺癌(NSCLC)细胞株的凋亡以及促凋亡蛋白(BIM)的表达,探讨吉非替尼对其的影响。方法采用Annexin PI单染法检测吉非替尼作用下各细胞株的凋亡,免疫印迹法检测BIM的表达。结果吉非替尼诱导NSCLC细胞株凋亡,其中对H1650、H1975突变株的诱导凋亡率高于A549细胞株(P<0.01),H1650的BIM表达随吉非替尼浓度的增加而增强,H1975的BIM表达较高,但受吉非替尼浓度变化的影响不明显,而A549的BIM表达需要更高浓度的吉非替尼作用。结论吉非替尼可以使NSCLC细胞表皮生长因子受体(EGFR)突变株细胞内的BIM表达增高,并且可能通过上调BIM的表达来诱导肿瘤细胞凋亡。
Objective To investigate the apoptosis and the expression of pro-apoptotic protein (BIM) in non-small cell lung cancer (NSCLC) cell lines and to explore the effect of gefitinib on it. Methods Annexin PI staining was used to detect the apoptosis of various cell lines with gefitinib. The expression of BIM was detected by Western blotting. Results Gefitinib induced the apoptosis of NSCLC cell lines. The apoptosis rate of H1650 and H1975 mutants was higher than that of A549 cell lines (P <0.01). The BIM expression of H1650 increased with the increase of gefitinib concentration, The BIM expression of H1975 was higher but not significantly affected by the change of gefitinib concentration, whereas the BIM expression of A549 required higher concentrations of gefitinib. Conclusion Gefitinib can increase the BIM expression in NSCLC cell epidermal growth factor receptor (EGFR) mutant cells and induce the apoptosis of tumor cells possibly by up-regulating the expression of BIM.