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以‘改良蒜’品种带蒜瓣基部的茎盘为外植体,在含有不同浓度大蒜紫斑病菌粗毒素的MS培养基上采取分步筛选法筛选大蒜抗病变异系,并诱导形成小鳞茎;对小鳞茎繁殖的幼苗叶面喷施病菌粗毒素进行抗病性鉴定。结果表明,大蒜紫斑病菌粗毒素对‘改良蒜’愈伤组织的诱导有显著抑制作用,粗毒素浓度越高,抑制作用越强,抗病愈伤组织诱导的适宜病菌粗毒素浓度为30%;在粗毒素浓度10%、20%和30%的培养基上依次分步培养筛选获得了抗紫斑病变异系。粗毒素接种抗性鉴定表明,抗性系幼苗的抗病性比对照增强。
The stem disks of the base of garlic cloves were used as explants in the improved garlic cultivars, and the resistant lines of garlic were screened by MS-MS on the MS medium containing different concentrations of V. versicolor to induce the formation of bulblets. The seedlings of bulblets were sprayed with the pathogen of pathogenicity on the leaves of the seedlings. The results showed that the crude toxin of Garcinia hyodysenteriae had a significant inhibitory effect on the induction of ’improved garlic’ callus. The higher the concentration of crude toxin was, the stronger the inhibitory effect was. The crude toxin concentration induced by resistant callus was 30%. In the crude toxin concentration of 10%, 20% and 30% of the medium in turn by step-by-step culture to obtain anti-purple spot mutation lines. Crude toxin inoculation resistance test showed that the resistance of the seedlings of disease resistance than the control.