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对水溶性的γ-聚谷氨酸(γ-PGA)进行了接枝改性,合成了两亲性γ-聚谷氨酸(γ-PGA)接枝衍生物,采用超声探头法制备胆甾醇基γ-PGA自组装胶束,并以卵清蛋白(OVA)作为模型蛋白,研究其载药和释药性能。结果表明,制备的两亲性胆甾醇基γ-PGA自组装胶束平均粒径为299.6+27.3nm,粒径的多分散系数较窄(0.17),且具有较低的细胞毒性;其疏水核-亲水壳的纳米微结构对蛋白药物显示了良好载药性能,对OVA载药量可达118.8μg/mg,包封率33.5%;体外释药结果显示,负载OVA的甾醇基γ-PGA自组装胶束能延缓蛋白的释放,释药速率与介质pH密切相关。
The water-soluble γ-polyglutamic acid (γ-PGA) was grafted and modified to produce amphiphilic γ-polyglutamic acid (γ-PGA) grafted derivatives. Ultrasound probe method was used to prepare cholesterol Based γ-PGA micelles. Ovalbumin (OVA) was used as a model protein to study the drug-loading and drug-releasing properties. The results showed that the amphiphilic cholesteryl γ-PGA self-assembled micelles had an average particle size of 299.6 ± 27.3 nm with a narrow polydispersity (0.17) and a low cytotoxicity. The hydrophobic core - The nanostructures of hydrophilic shell showed good drug-loading properties for protein drugs. The drug loading to OVA was 118.8 μg / mg and encapsulation efficiency was 33.5%. The in vitro drug release showed that OVA-loaded sterol-γ-PGA Self-assembled micelles can delay the release of protein, drug release rate and media pH are closely related.