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目的:探究体育运动是否影响大学生外周血T淋巴细胞Deltex 1(Dtx1)蛋白表达,以及对T细胞发育活性的影响。方法:将具有不同运动量及运动习惯的大学生志愿者分为3组(运动1组:>12 h/周,运动2组:>6 h/周,运动3组:<1 h/周),分别抽取外周血提取T淋巴细胞,MTT实验检测3组学生之间T淋巴细胞增殖能力差异,ELISA实验检测外周血IL-2与IL-10浓度差异,同时Western blotting实验检测3组学生之间T淋巴细胞内Dtx1蛋白表达差异。结果:3组成员T细胞增殖能力存在显著差异(P<0.05,运动1组>运动2组>运动3组);3组成员的外周血IL-2及IL-10浓度存在显著差异(P<0.05,运动1组>运动2组>运动3组);同时,3组成员的T淋巴细胞Dtx1蛋白表达存在显著差异(P<0.05,运动1组<运动2组<运动3组)。结论:体育运动可显著降低大学生T淋巴细胞内Dtx1蛋白的表达,显著增强T细胞的增殖发育能力以及活性。
Objective: To explore whether physical exercise affects Deltex 1 (Dtx1) protein expression in peripheral blood of college students and its effect on T cell developmental activity. Methods: The college students volunteers with different amount of exercise and exercise habits were divided into three groups (exercise 1:> 12 h / week, exercise 2:> 6 h / week, exercise 3: <1 h / T lymphocytes were extracted from peripheral blood, MTT assay was used to detect the difference of T lymphocyte proliferation between the three groups. ELISA was used to detect the concentration of IL-2 and IL-10 in peripheral blood. Western blotting was used to detect T lymphocyte Intracellular Dtx1 protein expression differences. Results: There were significant differences in T cell proliferation between the three groups (P <0.05, Exercise 1, Exercise 2, and Exercise 3). The concentrations of IL-2 and IL-10 in peripheral blood of 3 groups were significantly different (P < 0.05, Exercise 1, Exercise 2, and Exercise 3). Meanwhile, the expression of Dtx1 in T lymphocytes was significantly different among the 3 groups (P <0.05, Exercise 1, Exercise 2, Exercise 3). Conclusion: Physical exercise can significantly reduce the expression of Dtx1 protein in T lymphocytes and significantly enhance the ability of T cells to proliferate and develop.