Qingre Jianpi decoction(清热健脾汤)attenuates inflammatory responses by suppressing NOD-like receptor fam

• 作 者: ZHANG Jiayan KANG Xiuhong SUN Mengyun ZHANG Shengsheng
• 机 构: Department of Digestive System, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capit
• 出 处: 中医杂志（英文版）
• 发表日期: 2021年1期
• 关键词: Colitis ulcerative anti-inflammatory agents NLR family pyrin domain-containing 3

OBJECTIVE:To investigate the effects of Qingre Ji-anpi decoction(清热健脾汤,QRJPD)on dextran sulfate sodium(DSS)-induced colitis mice and ex-plore its mechanism.METHODS:All mice were randomly divided into six groups.Weight changes,disease activity index val-ues,and histological damage were detected.In-flammatory cytokines and immune cell infiltration were measured using enzyme-linked immunosor-bent assays(ELISA)and immunohistochemistry(IHC)method.The key protein expression levels of the NOD-like receptor family pyrin domain-contain-ing 3(NLRP3)inflammasome were detected by western blot analysis,IHC,and quantitative reverse transcription polymerase chain reaction.RESULTS:QRJPD played an anti-inflammatory role in the treatment of ulcerative colitis(UC),reduced the secretion of inflammatory cytokines,and inhib-ited the inflammatory infiltration of immune cells by suppressing DSS-induced activation of the NL-RP3 inflammasome.CONCLUSION:QRJPD exerts protective effects by inhibiting DSS-induced NLRP3 inflammasome activation.