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本试验以盆栽３年生新红星／海棠为试材，研究了苹果新根细胞分裂素的提取、纯化和高效形相色谱（ＨＰＬＣ）分析方法测定。苹果新根用８０％冷甲醇抽提，在ｐＨ３．０下用石油醚、乙酸乙酯和在ＰＨＳ．０～８．５下用正丁醇萃取，提取滚浓缩后用ＰＨ３．０的磷酸缓冲液做溶剂，加ＰＶＰ振荡吸附，最后用ＳｅＰ—ＰａｋＣ１８简柱进行纯化，以甲醇、水溶剂系统（ＰＨ３．５）在ＳｐｈｅｒｉｓｏｒｂＣ１８柱上进行分离，用紫外检测器在２６５ｍｍ波长下检测、鉴定．苹果新根中的ＣＴＫ种类主要有Ｚ、ＺＲ、ｄｉＨＺ。
In this study, potted three-year-old New Star / Begonia as test material, the apple new root cytokinin extraction, purification and high performance liquid chromatography (HPLC) analysis method. Fresh roots of apple are extracted with 80% cold methanol, petroleum ether, ethyl acetate and pH at pH 3.0. 0 ~ 8.5 with n-butanol extraction, extraction and rolling concentrated with PH3.0 phosphate buffer solvent, add PVP oscillation adsorption, and finally with SeP-PakC18 Jane column purification, methanol, water solvent system (PH3 .5) Separation on a Spherisorb C18 column, detection with UV detector at 265 mm wavelength, identification. CTK species in the new apple root are mainly Z, ZR, diHZ.