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目的探讨粘附在Cytodex-1微载体上的MC3T3-E1细胞是否可通过大梯度强磁场实现抗磁悬浮细胞培养,以及在该培养方式下不同模拟重力环境对细胞的生物学影响。方法鼠颅骨成骨细胞系MC3T3-E1接种于Cytodex-1微载体,将细胞置于大梯度强磁场的表观重力为μG,1 G和2 G位置,对应磁场强度分别为12 T,16 T和12 T,检测细胞形态、增殖、凋亡和周期。结果μG和1 G环境促进了MC3T3-E1细胞增殖,而2 G重力环境抑制了细胞增殖;大梯度强磁场环境诱导了细胞形态的改变、增加了细胞的caspase-3活性,影响了细胞周期。结论粘附在Cytodex-1微载体上的MC3T3-E1细胞在大梯度强磁场环境下可以实现抗磁悬浮培养,大梯度强磁场对细胞的形态和功能均具有较明显的影响。
OBJECTIVE: To investigate whether MC3T3-E1 cells adhering to Cytodex-1 microcarriers can achieve anti-magnetic suspension cell culture through a large gradient magnetic field and the biological effects of different simulated gravity environments on cells in this culture mode. Methods The mouse skull osteoblastic cell line MC3T3-E1 was seeded on Cytodex-1 microcarriers. The apparent gravities of the cells in the large gradient magnetic field were μG, 1G and 2G. The corresponding magnetic fields were 12 T, 16 T And 12 T, cell morphology, proliferation, apoptosis and cycle were detected. Results The proliferation of MC3T3-E1 cells was promoted by μG and 1G environment, while the cell proliferation was inhibited by 2G gravity environment. The changes of cell morphology were induced by strong magnetic field of large gradient and the activity of caspase-3 was increased and the cell cycle was affected. Conclusion The MC3T3-E1 cells adhering to the Cytodex-1 microcarriers can be cultured in anti-magnetic suspension under strong magnetic field with large gradient. The magnetic field with large gradient strongly affects the morphology and function of the cells.