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目的比较饮食诱导肥胖大鼠与肥胖抵抗大鼠三磷酸腺苷(adenosine triphosphate,ATP)生成量的差异。方法将健康雄性远交群(sprague dawley,SD)大鼠,随机分为基础饲料(control,CON)组和高脂饲料组,喂养2周后,将高脂饲料组按照体重增加量分为饮食诱导肥胖(diet-induced obesity,DIO)组和饮食诱导抵抗(diet-in-duced obesity resistance,DR)组。于喂养第10周末,麻醉处死动物,观察体重、摄食量、能量利用率以及肝脏、心脏、肌肉组织中ATP生成量的情况。结果 DIO组的体重一直高于DR组(均有P<0.05)。DIO组总能量摄入高于DR组和CON组(均有P<0.001),但DIO组与DR组能量利用率差异无统计学意义。DR组大鼠肝脏、心脏和肌肉组织中ATP生成量比DIO组分别高出12.8%,30.6%和11.6%。结论饮食诱导肥胖和肥胖抵抗大鼠的能量代谢存在差异,这种差异可能与主要能量器官中ATP的生成量有关。
Objective To compare the differences in the production of adenosine triphosphate (ATP) in diet-induced obesity rats and obesity-resistant rats. Methods Healthy male Sprague Dawley (SD) rats were randomly divided into control (CON) group and high fat diet group. After 2 weeks of feeding, the high fat diet group was divided into diet Diet-induced obesity (DIO) group and diet-induced obesity resistance (DR) group. At the end of the 10th week of feeding, animals were anesthetized and animals were sacrificed to observe the body weight, food intake, energy utilization, and ATP production in liver, heart and muscle tissue. Results The body weight of DIO group was always higher than that of DR group (all P <0.05). The total energy intake of DIO group was higher than that of DR group and CON group (both P <0.001), but there was no significant difference in energy utilization rate between DIO group and DR group. The ATP production in liver, heart and muscle tissue of DR group was 12.8%, 30.6% and 11.6% higher than that of DIO group respectively. Conclusion There is a difference in energy metabolism between diet-induced obesity and obesity-resistant rats, which may be related to the production of ATP in the main energy organs.