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干旱协迫下,抗旱(陕合6号)与干旱敏感(郑引1号)小麦品种叶片中的核糖核酸酶活性均有所增加,增加幅度与其相对含水量变化呈显著负相关。14C-Leu掺入后,样品粗提液经PAGE分离并染色,发现干旱处理叶片的两个低分子女(12kD、17kD)具RNase活性的蛋白质的放射性掺入总量低于对照,但占总蛋白的放射性比例高子对照,证明干旱胁迫下存在RNase的从头合成(denovosynthsis)。讨论了胁迫下RNase活性增加的原因。
Under drought co-operation, the activities of ribonucleases in drought-resistant wheat (Shaanhe 6) and drought-sensitive (Zhengyin 1) increased with the relative water content. After 14C-Leu incorporation, the crude extracts were separated by PAGE and stained. The results showed that the total radioactive incorporation of RNase activity of two low-molecular-weight female (12kD, 17kD) The radioactive high-sub-control of the protein demonstrated the denovosynthsis of RNase under drought stress. The reason for the increase of RNase activity under stress was discussed.