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目的评价无接触肿瘤分离技术(NTIT)降低胰头癌门静脉血内癌细胞播散的效果。方法38例胰头癌患者分为采用NTIT(N组,23例)和传统手术(C组,15例),肿瘤切除前、后分别抽取门静脉血,RT?PCR法测定静脉血内CK20mRNA表达。结果肿瘤切除前,N组门静脉血内CK20mRNA阳性率与C组相近(17.39%vs.13.34%)(P>0.05)。肿瘤切除后,N组门静脉血内CK20mRNA阳性率为21.73%,明显低于C组的73.34%(P<0.05)。结论采用NTIT能有效的减少胰头癌根治术中癌细胞脱落和播散。
Objective To evaluate the effect of non-contact tumor isolation (NTIT) in reducing the spread of cancer cells in the portal vein of pancreatic cancer. Methods Thirty-eight patients with pancreatic head cancer were divided into NTIT group (n = 23) and conventional surgery group (n = 15). The portal vein blood was collected before and after tumor resection. The expression of CK20 mRNA in venous blood was measured by RT-PCR. Results Before tumor resection, the positive rate of CK20 mRNA in portal vein of N group was similar to that of C group (17.39% vs.13.34%) (P> 0.05). After tumor resection, the positive rate of CK20 mRNA in group N was 21.73%, which was significantly lower than that in group C (73.34%, P <0.05). Conclusion The use of NTIT can effectively reduce the cancer cells in the radical pancreatic cancer shedding and dissemination.