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目的:优化草珊瑚总黄酮提取工艺。方法:应用响应面实验方法,利用Box-Behnken试验设计,考察乙醇回流法提取草珊瑚中的总黄酮的工艺因素。结果:草珊瑚总黄酮的乙醇回流法最佳提取条件为:浓度为60%的乙醇,温度为80℃的水浴回流,比例为1:22的固液比,该条件下草珊瑚中总黄酮的提取率约为6.532%。结论:响应面试验方法能够有效提高草珊瑚总黄酮的提取率。
Objective: To optimize the extraction technology of total flavonoids from Sarcandra glabra. Methods: Response surface methodology and Box-Behnken design were used to investigate the technological factors of extracting total flavonoids from Sarcandra glabra by ethanol reflux. Results: The optimal extraction conditions for total flavonoids from Sarcandra glabra were as follows: the concentration of 60% ethanol, the temperature of 80 ℃, the ratio of solid to liquid was 1:22, the total flavonoids The rate is about 6.532%. Conclusion: Response surface methodology can effectively improve the extraction rate of total flavonoids from Sarcandra glabra.