论文部分内容阅读
通过低温定向培养,从高纬、高寒野外厌氧环境样品和冬季沼气池底泥样品中分别选育出野生低温功能菌群和沼气池低温菌群。在13℃和10℃低温条件下,野生低温菌群产气率达到0.14 m3/d.m3和0.10 m3/d.m3,但所产沼气的甲烷含量较低,只有51.0%和48.0%。同样温度下,沼气池低温菌群的产气率只有0.06 m3/d.m3和0.04 m3/d.m3,但所产沼气的甲烷含量较高,达到62%和56%。两类菌群混合发酵可显著改善产气性能,相同温度下,产气率可达到0.15 m3/d.m3和0.11 m3/d.m3,高于两类菌群单独发酵,是常温菌群的3倍以上;混合低温菌群所产沼气的甲烷含量为57.0%和52.0%,介于野生低温菌群和沼气池低温菌群之间,比常温菌群提高19.0%和16.0%。研究结果表明,混合低温菌群具有较好的低温产气性能和广阔的应用前景。
Through low-temperature oriented cultivation, low temperature functional groups of wild and biogas digesters were selected from the anaerobic environment samples in high latitude and alpine fields and the sediment samples of biogas digesters in winter respectively. Under the conditions of 13 ℃ and 10 ℃, the biogas production rate of wild low-temperature bacteria reached 0.14 m3 / d.m3 and 0.10 m3 / d.m3, but the methane content of biogas produced was relatively low, only 51.0% and 48.0%. At the same temperature, the biogas digesters produced only 0.06 m3 / d.m3 and 0.04 m3 / d.m3 of gas, but the biogas produced had a higher methane content of 62% and 56%. The mixed fermentation of two kinds of bacteria can significantly improve the gas production performance. Under the same temperature, the gas production rate can reach 0.15 m3 / d.m3 and 0.11 m3 / d.m3, which is higher than the two types of bacteria alone, The methane content of biogas produced by mixed low-temperature bacteria group was 57.0% and 52.0%, which was between the low-temperature bacteria group of wild low-temperature bacteria group and biogas digester, 19.0% and 16.0% higher than that of normal temperature bacteria group. The results show that mixed low temperature bacteria have good low temperature gas production performance and broad application prospects.