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为了加深对安徽省铜陵市不同重金属富集程度的尾矿附近酸性废水中微生物多样性的了解,运用PCR-16S r DNA克隆文库技术对酸性废水中细菌及古菌的群落结构进行研究。在铜陵的5个样点采集了8个水样。系统发育分析结果表明,酸性废水中的细菌主要是Betaproteobacteria,Gammaproteobacteria,Alphaproteobacteria,Deinococcus-Thermus,Nitrospira,Firmicutes,Actinobacteria,Deltaproteobacteria,Bacteroidetes,Chloroflexi,古菌是Thermoplasma,Ferroplasma以及Thermogymnomonas。根据细菌和古菌的组成对样品进行聚类分析,结果表明,5个样品由于优势菌株Thermomonas和Meiothermus拥有较高的相似度。2个样品由于Acidithiobacillus和Leptospirillum占主导也拥有较高的相似度。剩余的样品多样性较高,拥有最高的香浓-微纳指数2.91。典型相关分析CCA的结果表明微生物的多样性组成与理化因子有密切的关系,如水的p H以及Hg2+、Pb2+、Cl-、SO2-4、Fe3+的含量。
In order to deepen the understanding of microbial diversity in acidic waste water near tailings with different concentrations of heavy metals in Tongling City, Anhui Province, the community structures of bacteria and archaea in acid wastewater were studied by PCR-16S rDNA cloning library. In Tongling five samples collected eight water samples. Phylogenetic analysis showed that the bacteria in acid wastewater were mainly Betaproteobacteria, Gammaproteobacteria, Alphaproteobacteria, Deinococcus-Thermus, Nitrospira, Firmicutes, Actinobacteria, Deltaproteobacteria, Bacteroidetes, Chloroflexi and Archaea were Thermoplasma, Ferroplasma and Thermogymnomonas. According to the composition of bacteria and archaea, the samples were clustered and analyzed. The results showed that the five samples had higher similarity due to the dominant strains Thermomonas and Meiothermus. Two samples also have a high degree of similarity due to the dominant Acidithiobacillus and Leptospirillum. The remaining sample is more diverse and has the highest Fragrance - Micro-Nano Index 2.91. Canonical correlation analysis CCA results show that the diversity of microbial composition and physical and chemical factors are closely related, such as water p H and Hg2 +, Pb2 +, Cl-, SO2-4, Fe3 + content.