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目的:观察左归丸和右归丸对老年大鼠海马糖皮质激素受体(GR)mRNA表达和下丘脑-垂体-肾上腺皮质(HPA)轴活性的影响,探索GRmRNA表达和HPA轴活性的关系。方法:以自然衰老SD雄性大鼠(24月龄)为肾虚衰老模型,大鼠随机分为老年对照组、左归丸组和右归丸组,每组35只,另设青年对照组(5月龄)35只,共4组。老年对照组大鼠常规喂养至24月龄。左、右归丸组大鼠自20月龄始,均按相当于左、右归丸生药7.5 g.kg-1(成人临床用量的15倍)自由饮用稀释药液,每周停药2天,连续4个月;采用原位杂交法检测大鼠海马组织GRmRNA表达,放免法检测大鼠血浆皮质酮(CORT)含量。结果:与青年对照组相比,老年对照组大鼠海马CA1区GRmRNA表达水平均显著下降(P<0.05),血浆CORT含量显著增高(P<0.05);与老年对照组比较,左归丸组和右归丸组大鼠海马CA1区GRmRNA表达水平显著升高(P<0.05),血浆CORT含量显著降低(P<0.05)。结论:左归丸和右归丸可能通过上调自然衰老SD雄性大鼠海马GRmRNA表达,加强对HPA轴的抑制作用,进而降低血浆CORT含量。
Objective: To observe the effect of Zuogui Pill and Yougui Pill on hippocampal glucocorticoid receptor (GR) mRNA expression and hypothalamic-pituitary-adrenal cortex (HPA) axis activity in aged rats and to explore the relationship between GR mRNA expression and HPA axis activity. Methods: Male SD rats aged 24 months were randomly divided into the aged control group, Zuoguiwan group and Yougui pill group, with 35 rats in each group. The rats in the youth control group (5) Month-old) 35, a total of 4 groups. The aged control rats were routinely fed to 24 months of age. Left and right Gui Wan group rats from 20 months of age, are equivalent to the left and right Guixi pill crude drug 7.5 g.kg-1 (15 times the clinical dose of adult) free to drink diluted solution, weekly withdrawal for 2 days For 4 consecutive months. The expression of GR mRNA in hippocampus of rats was detected by in situ hybridization, and the level of plasma corticosterone (CORT) was determined by radioimmunoassay. Results: Compared with the young control group, the expression of GR mRNA in the hippocampal CA1 region of the senile control group was significantly decreased (P <0.05) and the plasma CORT content was significantly increased (P <0.05). Compared with the aged control group, The mRNA expression of GR mRNA in hippocampal CA1 region of rats in Yougui Wan group was significantly increased (P <0.05), and the plasma CORT content was significantly decreased (P <0.05). Conclusion: Zuogui Pill and Yougui Pill may increase the expression of GR mRNA in the hippocampus of SD rats with natural aging and enhance the inhibitory effect on HPA axis, and then decrease the plasma CORT content.