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目的研究染料木黄酮(GST)在心血管中枢神经系统的作用。方法应用细胞外记录单位放电技术,在下丘脑脑片上观察GST对静息状态下的室旁核神经元放电的影响。结果①26个脑片分别灌流GST 10,50,100μmol·L~(-1)2 min,有25个脑片放电频率明显降低,且呈浓度依赖性;②用0.2 mmol·L~(-1) L-谷氨酸灌流脑片,7/7个脑片放电频率明显增加,表现为癫痫样放电,在此基础上加灌GST 50μmol·L~(-1)2 min,其癫痫样放电被抑制;③用G蛋白激活的内向整流型钾通道阻断剂四乙胺1 mmol·L~(-1)灌流脑片,约10 min后加入GST 50μmol·L~(-1),8/8个脑片的放电抑制效应被完全阻断;④用一氧化氮合酶抑制剂左旋硝基精氨酸甲酯50μmol·L~(-1)灌流脑片,7/7个脑片的放电频率增加,在此基础上加灌GST 50μmol·L~(-1)2 min,放电被抑制。结论GST可抑制下丘脑室旁核神经元自发放电,并抑制L-谷氨酸诱发的神经元癫痫样放电。这种抑制作用可能与激活G蛋白激活的内向整流型钾通道,促进K~+外流,从而引起细胞膜超极化有关;而与NO释放无关。GST可能通过降低心血管中枢的活动性而产生一定的心血管系统保护作用。
Objective To investigate the role of genistein (GST) in cardiovascular central nervous system. Methods The extracellular recording unit discharge technique was used to observe the effects of GST on resting paraventricular nucleus neurons in hypothalamus slices. RESULTS: Twenty-six brain slices were perfused with GST 10, 50 and 100 μmol·L -1 for 2 min, respectively. The frequency of discharge was significantly decreased in 25 slices and in a concentration-dependent manner. ② With 0.2 mmol·L -1 - Glutamic acid perfusion brain slices, 7/7 brain slices discharge frequency increased significantly, showing epileptiform discharge, on this basis, GST 50μmol·L -1 (-1) 2 min, epileptiform discharge was inhibited; ③ G-protein-activated inward rectifier potassium channel blocker tetraethylamine 1 mmol·L -1 perfusion brain slices, after about 10 min GST 50μmol·L -1, 8/8 brain The discharge inhibition effect of the tablets was completely blocked; ④ The perfusion of brain slices with L-nitro-N-methyl-arginine methyl ester 50μmol·L -1 increased the discharge frequency of 7/7 slices, On this basis, GST 50μmol·L -1 added for 2 min, the discharge was inhibited. Conclusion GST can inhibit spontaneous discharge of neurons in hypothalamic paraventricular nucleus and inhibit neuronal epileptiform discharge induced by L-glutamate. This inhibition may be related to the activation of G protein-activated inward rectifier potassium channel, promote K ~ + outflow, resulting in hyperpolarization of the cell membrane; and NO release has nothing to do. GST may have a cardiovascular protective effect by reducing the activity of the cardiovascular center.