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目的探讨再生室内联合应用牙髓干细胞(dental pulp stem cells,DPSCs)和转化生长因子-β3(transforming growth factor-β3,TGF-β3)修复兔面神经横断性损伤的可行性,比较各时间段不同药物干预后面神经的恢复情况。方法健康成年新西兰大白兔36只,随机分为DPSCs+TGF-β3组(实验组)、TGF-β3组和PBS组,每组各12只,3组均于兔左面颊部分离面神经上颊支并切断,利用硅胶管作为再生室桥接离断的神经上颊支,建立面神经上颊支横断伤模型。实验组在再生室内加入100ng/μL TGF-β3液和1×108/LDPSCs悬液0.1mL,TGF-β3组加入等量100ng/μL TGF-β3液,PBS组加入等量PBS液,术后4、12周比较3组兔面神经修复再生情况。结果4、12周时实验组神经纤维数[(1 034.58±94.28)、(1 425.92±188.98)根]和纤维直径[(2.05±0.14)、(7.18±0.40)μm]高于TGF-β3组[(935.00±44.60)、(1 053.42±135.26)根,(1.56±0.12)、(6.21±0.47)μm]和PBS组[(555.50±80.20)、(694.36±24.11)根,(1.26±0.08)、(3.64±0.56)μm](P<0.05);12周时3组再生神经纤维总数和纤维直径均高于4周时(P<0.05)。结论 TGF-β3与DPSCs联合应用可有效促进面神经再生,二者联合应用较单用TGF-β3修复效果好;DPSCs可作为种子细胞应用于面神经组织工程。
Objective To investigate the feasibility of repairing rabbit facial nerve transection induced by regenerative indoor dental pulp stem cells (DPSCs) and transforming growth factor-β3 (TGF-β3), and to compare the effects of different drugs Facial nerve recovery after intervention. Methods Thirty-six healthy adult New Zealand white rabbits were randomly divided into DPSCs + TGF-β3 group (experimental group), TGF-β3 group and PBS group, 12 rats in each group. And cut off, the use of silicone tube as a regeneration chamber bridging the broken off the nerve on the buccal branch, the facial nerve on the buccal and transverse transection model. In the experimental group, 100ng / μL TGF-β3 and 1 × 108 / LDPSCs suspension were added into the regeneration chamber, and the same volume of 100ng / μL TGF-β3 was added to the TGF-β3 group. After 12 weeks, the facial nerve regeneration and repair in 3 groups were compared. Results The number of nerve fibers [(1 034.58 ± 94.28), (1 425.92 ± 188.98)] and the diameter of fibers [(2.05 ± 0.14), (7.18 ± 0.40) μm] in the experimental group were significantly higher than those in the TGF-β3 group [(935.00 ± 44.60), (1 053.42 ± 135.26), (1.56 ± 0.12), (6.21 ± 0.47) μm] and PBS [(555.50 ± 80.20), (694.36 ± 24.11), (1.26 ± 0.08) , (3.64 ± 0.56) μm] (P <0.05). At 12 weeks, the total number of regenerated nerve fibers and the diameter of fibers in the three groups were all higher than those at 4 weeks (P <0.05). Conclusion Combined application of TGF-β3 and DPSCs can effectively promote facial nerve regeneration. The combined application of TGF-β3 and DPSCs is more effective than that of TGF-β3 alone. DPSCs can be used as seed cells in facial nerve tissue engineering.