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Maize is a globally important crop that was a classic model plant for genetic studies.Here,we report a 2.2 Gb draft genome sequence of an elite maize line,HuangZaoSi (HZS).Hybrids bred from HZS-improved lines (HILs) are planted in more than 60% of maize fields in China.Proteome clustering of six completed sequenced maize genomes show that 638 proteins fall into 264 HZS-specific gene families with the majority of contributions from tandem duplication events.Resequencing and comparative analysis of 40 HZS-related lines reveals the breeding history of HILs.More than 60% of identified selective sweeps were clustered in identity-by-descent conserved regions,and yield-related genes/QTLs were enriched in HZS characteristic selected regions.Furthermore,we demonstrated that HZS-specific family genes were not uniformly distributed in the genome but enriched in improvement/function-related genomic regions.This study provides an important and novel resource for maize genome research and expands our knowledge on the breadth of genomic variation and improvement history of maize.