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为培育适应山东省以及黄淮海区域生产的高产、高油酸花生新品种,以山东优异花生品种花育23号(HY23)和花育31号(HY31)为母本,以高油酸花生品种Sunoleic95R、DF12和开农176(KN176)为父本,配制了3个杂交组合HY23×DF12(SAAS-01)、HY31×KN176(SAAS-02)以及HY31×Sunoleic95R(SAAS-03),在3年时间内,进行了一次杂交、4次回交和1次自交,利用优化的CAPS和PCR产物测序法,以及新开发的花生FAD2基因KASP检测方法,对自交和回交后代进行检测。SAAS-01、SAAS-02和SAAS-03三个组合分别获得了FAD2A和FAD2B隐性纯合的高油酸基因型BC4F2种子1,18和26粒;另外还获得了一批基因型为Aabb、aa Bb、Aa Bb的BC_4F_2种子,这三种基因型的杂交后代可通过进一步自交获得纯合的高油酸材料。本研究获得了遗传背景为花育23号和花育31号的高油酸花生材料,为培育适合山东省种植的高产、高油酸花生新品种奠定了基础。
In order to cultivate new varieties of high yield and high oleic acid, which are suitable for the production of high yield and high oleic acid in Shandong and Huang-Huai-Hai areas, taking the peanut varieties HY23 and HY31 as the female parent, Sunoleic95R, DF12 and Kaiin176 (KN176) were used as parents to prepare three crosses HY23 × DF12 (SAAS-01), HY31 × KN176 (SAAS-02) and HY31 × Sunoleic95R (SAAS-03) The hybridization, four backcrosses and one selfing were carried out within the time, and the selfing and backcross progenies were detected by using optimized CAPS and PCR product sequencing methods and the newly developed KASP detection method of FAD2 gene in peanut. SAIC-01, SAAS-02 and SAAS-03, respectively. The results showed that 1, 18 and 26 seeds of BC4F2 were homozygous for the high oleic acid genotypes FAD2A and FAD2B respectively. A number of genotypes Aabb, aa Bb and Aa Bb BC_4F_2 seeds. The hybrid progeny of these three genotypes could be obtained by further selfing of homozygous high oleic acid. In this study, the high oleic acid peanut material with genetic background of Huayu 23 and Huayu 31 was obtained, which laid a foundation for cultivating new peanut varieties with high yield and high oleic content suitable for planting in Shandong Province.