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目的观察微波辐射对大鼠学习记忆能力和海马氨基酸类神经递质含量的影响。方法采用30 mW/cm2微波辐射56只Wistar雄性大鼠,于辐射后6 h、1 d、7 d、14 d和28 d,采用Morris水迷宫试验的平均逃避潜伏期检测大鼠的学习和记忆能力的改变;并于辐射后1 d、7 d、14 d和28 d,采用高效液相色谱法(HPLC)大鼠海马组织中天冬氨酸(Asp)、谷氨酸(Glu)、甘氨酸(Gly)及γ-氨基丁酸(GABA)4种氨基酸类神经递质含量的变化。结果微波辐射后6 h和7 d,大鼠的平均逃避潜伏期延长,与假辐射组相比有显著差异(P<0.05);辐射后1 d,辐射组Asp、Glu与GABA含量降低,与假辐射组相比有显著差异(P<0.01);辐射后7 d,辐射组Asp和Gly含量升高,与假辐射组相比有显著差异(P<0.05)。结论微波辐射可造成大鼠空间学习记忆能力下降及海马氨基酸类神经递质含量的改变,并且具有可恢复性。
Objective To observe the effects of microwave radiation on learning and memory ability and hippocampal amino acid neurotransmitters in rats. Methods Fifty-six male Wistar rats were irradiated with 30 mW / cm2 microwave at 6 h, 1 d, 7 d, 14 d and 28 d after radiation. The learning and memory abilities of rats were detected by the mean escape latency of Morris water maze test The changes of Asp, Glu and Gly (P <0.05) in the hippocampus of rats on day 1, day 7, day 14 and day 28 after radiation were detected by HPLC. Gly) and γ-aminobutyric acid (GABA). Results At 6 and 7 days after microwave irradiation, the mean escape latency of rats was longer than that of the sham radiation group (P <0.05). At 1 d after irradiation, the levels of Asp, Glu and GABA in the irradiation group were decreased, (P <0.01). At 7 days after irradiation, the levels of Asp and Gly in radiation group were significantly higher than those in radiation group (P <0.05). Conclusion Microwave radiation can decrease the spatial learning and memory capacity and the content of amino acid neurotransmitters in hippocampus, and have recoverability.