论文部分内容阅读
目的:探讨M2型肿瘤相关巨噬细胞(TAMs)在上皮性卵巢癌组织中的分布,及其与临床病理特征及淋巴管(LV)生成的关系。方法:本研究共纳入74例样本,41例卵巢癌为实验组,18例交界性及15例良性肿瘤为对照组,采用免疫组化方法,用CD68标记巨噬细胞(CD68+)、CD163标记M2型TAMs(CD163+)、D2-40标记LV,了解其在实验组和对照组的分布,及与卵巢癌临床病理特征的关系。结果:卵巢癌组CD68+细胞数、CD163+细胞数、LV数均多于交界性和良性肿瘤组(P<0.01)。CD68+细胞分布于癌巢和癌间质,CD163+细胞和LV主要分布于癌间质,癌间质中三者数量明显多于癌巢(P<0.01)。癌巢和癌间质中CD68+、CD163+细胞数及癌间质LV数与临床分期、组织学分化、病理类型、手术切缘累及淋巴结转移有关(P<0.01)。癌间质LV数与CD68+和CD163+细胞数相关(r=0.759,P<0.01;r=0.888,P<0.01)。结论:卵巢癌组织中TAMs主要为M2型,主要分布于癌间质。其细胞数与肿瘤的临床分期、组织分化、病理类型、手术切缘累及和淋巴结转移有关,提示TAMs可能通过促进淋巴管生成,利于肿瘤的淋巴转移。
Objective: To investigate the distribution of M2 tumor-associated macrophages (TAMs) in epithelial ovarian cancer and its relationship with clinicopathological features and lymphangiogenesis. METHODS: Totally 74 samples were enrolled in this study, 41 cases of ovarian cancer as experimental group, 18 cases of borderline and 15 cases of benign tumor as control group. Immunohistochemistry was used to detect CD68, CD68, Type TAMs (CD163 +), D2-40 labeled LV, to understand its distribution in the experimental group and control group, and with the clinicopathological features of ovarian cancer. Results: The number of CD68 + cells, CD163 + cells and LV in ovarian cancer group were more than those in borderline and benign tumor group (P <0.01). CD68 + cells were located in cancer nests and cancer stroma. CD163 + cells and LV were mainly located in cancer stroma. The number of CD68 + cells was significantly higher in cancer stroma than in cancer nests (P <0.01). The number of CD68 + and CD163 + cells in cancer nests and cancer interstitium and the number of LV in cancer were related to clinical stage, histological differentiation, pathological type and lymph node metastasis (P <0.01). The number of LVs was correlated with the number of CD68 + and CD163 + cells (r = 0.759, P <0.01; r = 0.888, P <0.01). Conclusion: TAMs in ovarian cancer are mainly M2, which are mainly distributed in the cancer stroma. The number of cells and the clinical stage of the tumor, tissue differentiation, pathological type, surgical margin involvement and lymph node metastasis, suggesting that TAMs may promote lymphangiogenesis, which is conducive to tumor lymphatic metastasis.