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[Objective] To establish a rapid propagation system for the tissue culture seedlings of Asclepsus curassavica,in order to provide theoretical basis for the fine variety breeding.[Method] Taking the seeds of wild A.curassavica as research materials,induction effects of different concentrations of hormone combinations on the germination,differentiation,and rooting of A.curassavica were compared,based on the aseptic germination.[Result] A.curassavica seeds showed good germination on MS culture medium.On the culture medium(4) of MS+0.2 mg/L 6-BA+0.5 mg/L NAA,bud showed good proliferation.The culture medium(7) of MS+0.5 mg/L NAA had good induction effect on the root.[Conclusion] Technique of tissue culture and rapid propagation were suitable for the seedling cultivation of A.curassavica.
[Objective] To establish a rapid propagation system for the tissue culture seedlings of Asclepsus curassavica, in order to provide theoretical basis for the fine variety breeding. [Method] Taking the seeds of wild A.curassavica as research materials, induction effects of different concentrations of hormone combinations on the germination, differentiation, and rooting of A. curassavica were compared, based on the aseptic germination. [Result] A. curassavica seeds showed good germination on MS culture medium. On the culture medium (4) of MS + 0.2 mg / L 6-BA + 0.5 mg / L NAA, bud showed good proliferation. The culture medium (7) of MS + 0.5 mg / L NAA had good induction effect on the root. [Conclusion] Technique of tissue culture and rapid propagation were suitable for the seedling cultivation of A.curassavica.