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目的研究短指软珊瑚Sinulariasp.的化学成分。方法利用硅胶色谱、Sephadex LH-20凝胶色谱、HPLC等手段对化学成分进行分离纯化;通过理化性质、波谱分析方法结合文献对照,鉴定化合物的结构。结果从短指软珊瑚甲醇提取物中,共分离鉴定了11个单体化合物:胆甾醇(1)、(22E)-麦角甾-5,22-二烯-3β-醇(2)、胆甾-5,20-二烯-3β-醇(3)、麦角甾-5,24(28)-二烯-3β-醇(4)、柳珊瑚甾醇(5)、3β-羟基胆甾-5-烯-7-酮(6)、3β-羟基麦角甾-5,24(28)-二烯-7-酮(7)、(22E)-3β-羟基麦角甾-5,22-二烯-7-酮(8)、3β-羟基麦角甾-5-烯-7-酮(9)、(22E)-3β-羟基胆甾-5,22-二烯-7-酮(10)、鲨肝醇(11)。结论化合物6～10为首次从该属软珊瑚中分离得到。化合物7在10μg.mL-1浓度水平,对K562肿瘤细胞株的抑制率为22.74%,对HeLa肿瘤细胞株的抑制率为9.98%。
Aim To study the chemical constituents of Sinulariasp. Methods The chemical constituents were separated and purified by silica gel chromatography, Sephadex LH-20 gel chromatography and HPLC. The structures of the compounds were identified by physico-chemical and spectral analysis combined with literature references. Results A total of 11 monomer compounds were isolated and identified from the methanolic extracts of the short-legged soft corals. Cholesterol (1), (22E) -ergosta-5,22-dien-3β- -5,20-dien-3β-ol (3), ergosta-5,24 (28) -dien-3β- (7), (22E) -3β-hydroxyeptol-5,22-diene-7 -hydroxy ergost-5-en-7-one (9), (22E) -3β-hydroxycholesta-5,22-dien-7-one (10), batyl alcohol (11). Conclusion Compounds 6 ~ 10 were isolated from this genus of soft corals for the first time. Compound 7 had a inhibitory rate of 22.74% for K562 tumor cell line and 9.98% for HeLa tumor cell line at a concentration of 10 μg.mL-1.