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研究了低温胁迫下黄瓜花叶病毒与三生烟互作后CMV cp基因序列的变化规律,并分析了植物RNA病毒的进化机制。用黄瓜花叶病毒北京甘蓝分离物(CMV-BG)侵染三生烟不同单株,置于不同温度条件下(常温和低温)培养30 d后,对CMV cp基因进行克隆、序列测定和多态性分析。结果表明,侵染低温组植物CMV的cp基因多态性(Pi=0.001 76)高于常温组(Pi=0.001 14),IFEL分析检测到低温组第48个氨基酸位点为正选择位点,初步表明低温胁迫有助于提高CMV-BG cp基因序列的多态性,温度胁迫是植物RNA病毒与宿主互作过程中基因序列变异的一种重要的作用力。
The variation of CMV cp gene sequence after cucumber mosaic virus interaction with tobacco under low temperature stress was studied. The evolution mechanism of plant RNA virus was also analyzed. The CMV cp gene was cloned and sequenced with different cucumber plants (CMV-BG) at different temperatures (normal temperature and low temperature) for 30 days after being infected with CMV-BG Analysis of the state. The results showed that the cp gene polymorphism (Pi = 0.001 76) of CMV infected with low temperature group was higher than that of normal temperature group (Pi = 0.001 14). IFEL analysis detected that the 48th amino acid site of low temperature group was the positive selection site, Preliminary results showed that low temperature stress could improve the polymorphism of CMV-BG cp gene sequence. Temperature stress is an important force of gene sequence variation during the interaction between plant RNA virus and host.