Identification and Function of Acid-sensing Ion Channels in RAW 264.7 Macrophage Cells

• 作 者: Lan NI Peng FANG Zhuang-li HU Hai-yun ZHOU Jian-guo CHEN Fang WANG You JIN
• 机 构: Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, W
• 出 处: 华中科技大学学报（医学英德文版）
• 发表日期: 2018年3期
• 关键词: acid-sensing ion channels (ASICs) RAW 264.7 cells inflammation apoptosis migrati

Activation of acid-sensing ion channels (ASICs) plays an important role in neuroinflammation.Macrophage recruitment to the sites of inflammation is an essential step in host defense.ASIC 1 and ASIC3 have been reported to mediate the endocytosis and maturation of bone marrow derived macrophages.However,the expression and inflammation-related functions of ASICs in RAW 264.7 cells,another common macrophage,are still elusive.In the present study,we first demonstrated the presence of ASIC 1,ASIC2a and ASIC3 in RAW 264.7 macrophage cell line by using reverse transcriptase polymerase chain reaction (RT-PCR),West blotting and immunofluorescence experiments.The non-specific ASICs inhibitor amiloride and specific homomeric ASIC 1 a blocker PcTx 1 reduced the production of iNOS and COX-2 by LPS-induced activating RAW 264.7 cells.Furthermore,not only amiloride but also PcTx 1 inhibited the migration and LPS-induced apoptosis of RAW 264.7 cells.Taken together,our findings suggest that ASICs promote the inflammatory response and apoptosis of RAW 264.7 cells,and ASICs may serve as a potential novel target for immunological disease therapy.