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[目的]研究纳米二氧化硅(nano-SiO_2)对人肺腺癌A549细胞的毒性作用。[方法]将A549细胞随机分成6组,即对照组和实验组(5、10、25、50、100 mg/L nano-SiO_2组)。实验组分别用不同浓度的nano-SiO(210~20 nm粒径)刺激,MTT法检测细胞活性,比色法测定乳酸脱氢酶(LDH)活性,透射电子显微镜观察细胞微观结构,流式细胞仪检测nano-SiO_2对细胞周期以及细胞凋亡的影响。[结果]与对照组相比:随着nano-SiO_2浓度升高,A549细胞存活率呈降低趋势;LDH活性增加(P<0.01或P<0.05),在浓度为0、5、10、25 mg/L时呈浓度依赖关系,但在nano-SiO_2浓度为50 mg/L时,LDH活力降低,100 mg/L时增高。nano-SiO_2浓度的升高可导致细胞膜表面绒毛凸起减少,细胞器空泡样变增加,次级溶酶体增多;细胞凋亡率增加,坏死率增加;细胞在G0/G1期阻滞,增殖受抑制。[结论]nano-SiO_2作用于A549细胞可被细胞摄取,使细胞器结构功能受损,从而抑制细胞增殖、诱导细胞凋亡,引起细胞周期紊乱。
[Objective] To study the toxic effects of nano-SiO 2 on human lung adenocarcinoma A549 cells. [Methods] A549 cells were randomly divided into 6 groups: control group and experimental group (5,10,25,50,100 mg / L nano-SiO 2 group). The experimental groups were stimulated with different concentrations of nano-SiO (210 ~ 20 nm), MTT assay was used to measure the activity of lactate dehydrogenase (LDH), the microstructure of cells was observed by transmission electron microscopy, flow cytometry The effect of nano-SiO 2 on cell cycle and apoptosis was examined. [Results] Compared with the control group, the survival rate of A549 cells decreased as the concentration of nano-SiO 2 increased, and the activity of LDH increased (P <0.01 or P <0.05). At the concentration of 0,5,10,25 mg / L in a concentration-dependent manner, but at a nano-SiO 2 concentration of 50 mg / L, the activity of LDH decreased and increased at 100 mg / L. The concentration of nano-SiO 2 could lead to the decrease of villi on the surface of cell membrane, vacuolization of organelles and increase of secondary lysosomes, the increase of apoptosis rate and the increase of necrosis rate. The cells arrested and proliferated in G0 / G1 phase Suppressed. [Conclusion] The nano-SiO 2 could be taken up by A549 cells, which impaired the function of organelles, inhibited cell proliferation, induced cell apoptosis and caused cell cycle disorders.