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目的探讨十全大补汤对小鼠肝癌H22抑瘤作用及其机理。方法复制小鼠肝癌H22模型,实验分为模型组,5-FU组,中药高、中、低剂量组,分别计算抑瘤率;用免疫组化法检测凋亡指数。结果中药高、中、低剂量组均有一定的抑制作用,以中剂量组为最佳,抑瘤率为54.8%,且增加凋亡指数;与5-FU组比较,均差异显著。结论中药(十全大补汤)高、中、低剂量组对小鼠肝癌H22有明显抑瘤作用,以中剂量组为最佳,增加凋亡指数可能是其抑制肿瘤的主要机理之一。
Objective To investigate the anti-tumor effect of Shiquan Dabu Tang on hepatoma H22 in mice and its mechanism. Methods The mouse H22 liver cancer model was replicated. The experiment was divided into model group, 5-FU group, high, medium and low dose groups of traditional Chinese medicine. The rate of tumor inhibition was calculated and the apoptosis index was detected by immunohistochemistry. RESULTS: The high, medium and low doses of traditional Chinese medicine all had some inhibitory effects. The best dose was medium dose group, the tumor inhibition rate was 54.8%, and the apoptotic index was increased. Compared with the 5-FU group, the difference was significant. Conclusion Chinese medicine (Shiquan Dabu Decoction) high, middle and low dose groups have significant anti-tumor effect on hepatoma H22 in mice, and the middle dose group is the best. Increasing the apoptosis index may be one of the main mechanisms of tumor inhibition.