Interaction of Lanthanide Ions With the Sarcoplasmic Reticulum (Ca~(2+)+Mg~(2+))-ATPase

• 作 者: 李新民 倪嘉缵 王平原 陈建文 黄芬
• 机 构: Laboratory of Rare Earth Chemistry & Physics, Changchun Institute of Applied Chemistry, Academia Sin
• 出 处: Progress in Natural Science
• 发表日期: 1994年05期
• 关键词: ATPase reticulum phosphatidic inhibited purified lanthanide stimulate activate v

The effect and mechanism of action of lanthanide ions on sarcoplasmic reticulum (SR) (Ca2++Mg2+)-ATPase activity are investigated. Low concentrations of Gd3+ stimulate the SR ATPase activity and the activity is inhibited with the increasing of the concentrations of Gd3+. Gd3+ inhibits the purified ATPase activity. When the purified ATPase is reconstituted with phosphatidic acid (PA) or cardiolipin (CL), low concentrations of Gd3+ activate its activity, whereas the activity will be inhibited if the enzyme is reconstituted with phosphatidylcholine (PC), phosphatidylserine (PS) and the mixture of phosphatidylcholine and phosphatidylethanolamine (PC/PE). Furthermore, different actions of Tb3+ on Ca2+-binding sites of the SR and purified ATPase have been observed.

The effects and mechanism of action of lanthanide ions on sarcoplasmic reticulum (SR) (Ca2 ++ Mg2 +) - ATPase activity are investigated. Low concentrations of Gd3 + stimulate the SR ATPase activity and the activity is inhibited with the increasing of the concentrations of Gd3 +. Gd3 + inhibits the purified ATPase activity. When the purified ATPase is reconstituted with phosphatidic acid (PA) or cardiolipin (CL), low concentrations of Gd3 + activate its activity, while the activity will be inhibited if the enzyme is reconstituted with phosphatidylcholine (PC), Phosphhatidylserine (PS) and the mixture of phosphatidylcholine and phosphatidylethanolamine (PC / PE). Further, different actions of Tb3 + on Ca2 + -binding sites of the SR and purified ATPase have been observed.