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目的分析哮喘小鼠模型肺组织及骨髓中CD25及FOXP3的表达,以及地塞米松的干预作用。方法 BALB/c小鼠随机分为正常对照、哮喘及地塞米松干预组,通过HE染色观察肺组织的病理变化,应用Western blot、RT-PCR方法检测肺组织FOXP3及CD25的表达,RT-PCR方法检测哮喘及地塞米松干预组肺及骨髓FOXP3 mRNA表达。结果哮喘及地塞米松干预组肺组织FOXP3表达强于正常对照组(P<0.05),地塞米松干预促进FOXP3表达(P<0.05);哮喘及地塞米松干预组肺组织CD25表达强于正常对照组(P<0.05),而两组之间无明显差异(P>0.05);哮喘及地塞米松干预组肺组织及骨髓细胞均有FOXP3 mRNA表达,且地塞米松组强于哮喘组(P<0.05)。结论哮喘小鼠肺内CD25及FOXP3表达增强,地塞米松会促进其表达;哮喘小鼠骨髓有FOXP3表达,地塞米松促进其表达。
Objective To analyze the expression of CD25 and FOXP3 in lung and bone marrow of asthmatic mice and the intervention of dexamethasone. Methods BALB / c mice were randomly divided into normal control, asthma and dexamethasone intervention groups. The pathological changes of lung tissues were observed by HE staining. The expression of FOXP3 and CD25 in lung tissues was detected by Western blot and RT-PCR. Methods The expression of FOXP3 mRNA in lung and bone marrow of asthma and dexamethasone intervention groups was detected. Results The expression of FOXP3 in lung tissue of asthma and dexamethasone intervention group was stronger than that of normal control group (P <0.05), and the expression of FOXP3 was promoted by dexamethasone intervention (P <0.05). The expression of CD25 in lung tissue of asthma and dexamethasone intervention group was stronger than that of normal group (P <0.05), but there was no significant difference between the two groups (P> 0.05). The expression of FOXP3 mRNA in lung and bone marrow cells of asthma and dexamethasone intervention groups was stronger than dexamethasone group P <0.05). Conclusion The expression of CD25 and FOXP3 in the lungs of asthmatic mice is enhanced, and dexamethasone promotes its expression. The expression of FOXP3 in bone marrow of asthmatic mice and dexamethasone are promoted.