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代谢型谷氨酸受体5(mGlu5)与神经元存活及脑肿瘤发生关系密切.近年发现,mGlu5在肝组织中有表达,并且在肝的病理过程中发挥重要的调节作用.而mGlu5是否在肝癌中起作用,目前尚未见报道.本研究选用代谢型谷氨酸受体特异性激动剂二羟基苯甘氨酸(dihydroxyphenylglycine,DHPG)处理肝癌细胞HepG2,从而探讨激活mGlu5对肝癌细胞生长的影响及其机制.结果显示,激活mGlu5能够促进HepG2细胞生长,并激活ERK/JNK通路,抑制p38通路,进而激活转录因子CREB/Elk1和NF-κB.本文揭示了MAPK通路可能参与mGlu5对肝癌细胞生长的调控,为临床提供以mGlu5作为药物靶位点的肝癌治疗新思路.
In recent years, mGlu5 has been expressed in liver tissue and plays an important regulatory role in the pathological process of liver.MGlu5 is closely related to the survival of neurons and brain tumors.MGlu5 And has not been reported yet.In this study, HepG2 cells were treated with dihydroxyphenylglycine (DHPG), a specific glutamate receptor agonist, to investigate the effect of mGlu5 on hepatocellular carcinoma Mechanism.The results showed that activation of mGlu5 can promote the growth of HepG2 cells and activate the ERK / JNK pathway, and inhibit the p38 pathway, thereby activating the transcription factors CREB / Elk1 and NF-κB.This paper reveals that MAPK pathway may be involved in the regulation of mGlu5 on the growth of hepatocellular carcinoma cells , Providing a new idea for the clinical treatment of liver cancer with mGlu5 as a drug target site.