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目的明确四川江油地区栽培乌头霜霉病病原菌乌头霜霉Peronospora aconiti rDNA-ITS和28 S rDNA D1/D2区序列,为病害诊断和防治提供理论基础。方法从病株收集病原菌分生孢子及菌丝,提取DNA,扩增rDNA-ITS和28 S rDNA D1/D2片段序列,进行测序分析,并构建邻接(neighbor-joining,NJ)发育树分析病原菌种类。结果检测出的病原菌rDNA-ITS序列与NCBI数据库中霜霉属P.pulveracea、P.aparines相似度为94%,28 S rDNA D1/D2区序列与霜霉属P.pulveracea、P.ficariae、P.bulbocapni相似度达97%。结论分子rDNA-ITS和28 S rDNA D1/D2区序列鉴定的结论和形态学鉴定的结论一致,乌头霜霉病病原菌为霜霉科霜霉属乌头霜霉Peronospora aconiti Yu,其rDNA-ITS和28 S rDNA D1/D2区序列可用于该病原物的鉴定。
Objective To determine the sequence of Peronospora aconiti rDNA-ITS and 28 S rDNA D1 / D2 in the pathogen of aconite and downy mildew in Jiangyou, Sichuan Province, and to provide a theoretical basis for disease diagnosis and prevention. Methods The conidia and mycelium of pathogen were collected from the diseased plants. DNA was amplified, and the rDNA-ITS and 28S rDNA D1 / D2 sequences were amplified and sequenced. The pathogen species were analyzed by neighbor-joining (NJ) . Results The sequence of rDNA-ITS was 94% identical to P.pulveracea and P.aparines in the NCBI database. The sequence of 28S rDNA D1 / D2 was similar to P.pulveracea, P.ficariae, P .bulbocapni similarity of 97%. Conclusion The conclusion of molecular sequence identification of rDNA-ITS and 28 S rDNA D1 / D2 region is consistent with the morphological identification. The pathogen of downy mildew is Wuyongpenglung (Peronospora aconiti Yu) with rDNA-ITS And 28 S rDNA D1 / D2 region sequences can be used for the identification of the pathogen.