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于１９９６—１９９７年春、秋渔汛季节，在山东青岛、日照沿海采集日本鬼鲉，分离毒腺，提取毒腺粗提液，对大鼠后肢进行肌肉注射，采用组织学、组织化学、放射免疫学方法观察和检测局部皮肤和肌肉的组织结构改变、注射局部肌细胞内的琥珀酸脱氢酶（ＳＤＨ）及神经肌肉连接处的已酰胆碱酯酶（ＡｃｈＥ）活性变化以及神经肽──Ｐ物质和生长抑素含量的改变。结果表明，毒腺粗提液局部注射可造成大鼠肢体麻痹；局部皮肤和肌肉组织明显肿胀，肌细胞有损伤，其内ＳＤＨ活性显著降低，肌细胞及其间质水肿、血管扩张、伴有出血现象；神经肌肉连接处ＡｃｈＥ活性显著增强；局部肌肉组织中Ｐ物质含量显著升高，而生长抑素含量显著降低。提示毒棘螫伤造成的剧烈疼痛和肿胀可能与Ｐ物质有关，造成肢体麻痹可能与肌细胞损伤及神经肌肉接头处乙酰胆碱分解过程增强有关。
In the spring of 1996-1997, autumn and autumn seasons, ginkgo was collected along the coast of Qingdao and Rizhao in Shandong Province, and the venom gland was isolated. The venom gland extract was extracted and the hind limbs of rats were injected intramuscularly. The histological, histochemical and radioimmunological methods were used to observe And the detection of local skin and muscle tissue changes, injection of local muscle cell succinate dehydrogenase (SDH) and neuromuscular junction of acetylcholinesterase (AchE) activity changes and neuropeptide ─ ─ P material and Somatostatin content changes. The results showed that local injection of venom gland extract could cause limb paralysis in rats; local skin and muscle tissue were obviously swollen, muscle cells were damaged, SDH activity was significantly decreased, muscle cells and interstitial edema, vasodilation and hemorrhage Phenomenon; AchE activity of neuromuscular junction significantly increased; local muscle tissue substance P content was significantly increased, while the somatostatin content was significantly reduced. Prompt poisonous spine sting injury caused by severe pain and swelling may be related to substance P, resulting in limb paralysis may be muscle cell damage and neuromuscular junction acetylcholine decomposition process enhancement.