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从大鼠脾细胞提取DNA聚合酶,经DEAE纤维素分离可分为DNA聚合酶α及β,DNA聚合酶的活性与衰老无关,而DNA聚合酶α催化DNA合成的忠实性在衰老过程中明显降低,当DNA合成的反应体系中缺失三种脱氧核苷酸时,断乳鼠、青年鼠及老年鼠脾细胞DNA聚合酶α的活性分别相当于4种脱氧核苷酸均存在时的4.0％,7.6％及13.9％。DNA聚合酶α催化DNA合成的忠实性还受二价阳离子的影响,当以M■~(++)代替Mg~(++)时,其忠实性降低。DNA聚合酶β催化DNA合成的忠实性与衰老无关。
DNA polymerase was extracted from rat spleen cells and separated into DNA polymerase alpha and beta by DEAE cellulose. The activity of DNA polymerase was not related to aging, while DNA polymerase alpha catalyzed the DNA synthesis faithful during aging Decreased when the DNA synthesis of the reaction system in the absence of three deoxynucleotides, weaned mice, young mice and elderly mice spleen DNA polymerase a activity equivalent to four kinds of deoxynucleotides are present 4.0% , 7.6% and 13.9% respectively. The faithfulness of DNA polymerase α catalyzing DNA synthesis is also affected by divalent cations, and its loyalty decreases when Mg ~ (++) is substituted for Mg ~ (++). DNA polymerase β catalyzes the loyalty of DNA synthesis has nothing to do with aging.