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目的了解山西地区淋球菌流行株的质粒谱,探讨淋球菌质粒谱与耐药谱间的关系。方法采用碱裂解法提取山西地区54株淋球菌的质粒,分析其质粒谱型。结果 54株淋球菌中,51株有可靠的分析结果,其中49株检测出携带有质粒,检出率96.08%。以7.4kb+4.2kb,39.5kb+7.4kb+4.2kb及42.5kb+39.5kb+7.4kb+4.2kb三种类型居多,占82.35%。结论质粒图谱显示山西地区的质粒谱型有别于国内其他地区,具有地区特殊性,产青霉素酶淋球菌(PPNG)与7.4kb质粒密切相关。
Objective To understand the plasmid spectrum of Neisseria gonorrhoeae strains in Shanxi Province and to explore the relationship between plasmid spectrum and drug resistance spectrum of Neisseria gonorrhoeae. Methods Plasmids of 54 strains of Neisseria gonorrhoeae from Shanxi were extracted by alkaline lysis, and their plasmid profiles were analyzed. Results Of 54 strains of Neisseria gonorrhoeae, 51 strains had reliable results. Among them, 49 strains were detected with plasmid and the detection rate was 96.08%. The majority of the three types are 7.4kb + 4.2kb, 39.5kb + 7.4kb + 4.2kb and 42.5kb + 39.5kb + 7.4kb + 4.2kb, accounting for 82.35%. Conclusion The plasmid map shows that the plasmid profile of Shanxi Province is different from other regions in China and has the regional specificity. The penicillinase-producing Neisseria gonorrhoeae (PPNG) is closely related to the 7.4 kb plasmid.