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[目的]探讨结缔组织生长因子(CTGF)与Ⅱ型肺泡细胞表面抗原(KL-6)在染矽尘大鼠血清、支气管肺泡灌洗液(BALF)及肺组织中的表达及意义。[方法]将49只Wistar雄性大鼠随机分为对照组和染尘后7、14、21、28、60、90 d共7个组,每组7只,染尘组采用非气管暴露法灌注二氧化硅悬浮液(100 mg/m L),对照组以同样方法灌注生理盐水,取其肺组织并采集血清及BALF。采用免疫组织化学法测定肺组织中CTGF和KL-6的表达水平;采用酶联免疫吸附法(ELISA)检测血清及BALF中CTGF、KL-6的质量浓度。[结果]免疫组化结果显示,染尘大鼠肺组织中CTGF和KL-6呈阳性表达,且染尘组的平均光密度值均高于对照组,差异有统计学意义(P<0.05);染尘后随时间的延长,CTGF表达呈先增后降的趋势,在染尘后28 d达到最高峰,差异有统计学意义(P<0.05);KL-6呈持续上升趋势,且差异有统计学意义(P<0.05)。ELISA结果显示,染尘大鼠血清及BALF中CTGF质量浓度的变化趋势基本与其在肺组织中的一致,但是在染尘后21 d达到峰值;KL-6在血清及BALF中的变化也基本与肺组织中一致。[结论]CTGF和KL-6与肺纤维化有密切的关系,在矽肺形成过程中发挥了作用。
[Objective] To investigate the expression and significance of connective tissue growth factor (CTGF) and type Ⅱ alveolar cell surface antigen (KL-6) in serum, bronchoalveolar lavage fluid (BALF) [Methods] Forty-nine Wistar male rats were randomly divided into control group and 7 groups at 7, 14, 21, 28, 60 and 90 days after dying. Seven rabbits in each group were drenched with non-tracheal exposure Silica suspension (100 mg / m L). The control group was perfused with saline in the same manner. The lung tissue was taken and serum and BALF were collected. The expression of CTGF and KL-6 in lung tissue were detected by immunohistochemical method. The concentrations of CTGF and KL-6 in serum and BALF were detected by enzyme-linked immunosorbent assay (ELISA). [Results] The results of immunohistochemistry showed that the expression of CTGF and KL-6 in the lung tissue of the infected rats were significantly higher than those in the control group (P <0.05) (P <0.05). The expression of CTGF increased first and then decreased, and reached the peak at 28 d after dying. The difference was statistically significant There was statistical significance (P <0.05). The results of ELISA showed that the trend of CTGF concentration in the serum and BALF of the exposed rats was almost the same as that in the lung tissue, but reached its peak on the 21st day after the dust exposure. The change of KL-6 in serum and BALF was also similar to Consistent in lung tissue. [Conclusion] CTGF and KL-6 are closely related to pulmonary fibrosis and play a role in the formation of silicosis.