Effect of ephrin-B2 on the expressions of angiopoietin-1 and-2 after focal cerebral ischemia/reperfu

• 作 者: Hui Xiao Qing Huang Jia-qi Wang Qing-qing Deng Wen-ping Gu
• 机 构: Department of Neurology,Changsha Municipal Central Hospital,Department of Neurology,Xiangya Hospital
• 出 处: Neural Regeneration Research
• 发表日期: 2016年11期
• 关键词: angiopoietin osmotic angiogenesis cortex occlusion unclear staining polyclonal r

Ephrin-B2 has been shown to participate in angiogenesis,but the underlying mechanisms involved remain unclear.In this study,a rat model of focal cerebral ischemia was prepared by focal middle cerebral artery occlusion,followed by 24-hour reperfusion.Then,ephrin-B2 protein was administered intracerebroventricularly for 3 consecutive days via a micro-osmotic pump.Western blot assay and quantitative real-time reverse transcription PCR demonstrated the expression levels of angiopoietin-1(Ang-1) mRNA and protein in the penumbra cortex of the ephrin-B2 treated group were decreased at day 4 after reperfusion,and increased at day 28,while the expression levels of angiopoietin-2(Ang-2) were highly up-regulated at all time points tested.Double immunofiuorescent staining indicated that Ang-1 and Ang-2 were both expressed in vascular endothelial cells positive for CD31.These findings indicate that ephrin-B2 influences the expressions of Ang-1 and Ang-2 during angiogenesis following transient focal cerebral ischemia.

Ephrin-B2 has been shown to participate in angiogenesis, but the underlying mechanisms involved remain unclear. In this study, a rat model of focal cerebral ischemia was prepared by focal middle cerebral artery occlusion, followed by 24-hour reperfusion. Chen, ephrin- B2 protein was administered intracerebroventricularly for 3 consecutive days via a micro-osmotic pump. Western blot assay and quantitative real-time reverse transcription PCR demonstrated the expression levels of angiopoietin-1 (Ang-1) mRNA and protein in the penumbra cortex of the ephrin -B2 treated group were decreased at day 4 after reperfusion, and increased at day 28, while the expression levels of angiopoietin-2 (Ang-2) were highly up-regulated at all time points tested. Double immunofiuorescent staining indicated that Ang-1 and Ang-2 were both expressed in vascular endothelial cells positive for CD31. These findings predict that ephrin-B2 influences the expressions of Ang-1 and Ang-2 during angiogenesis the following transient focal cerebral ischemia.