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目的评估银染法鉴定铜绿假单胞菌生物被膜的效果。方法体外平板法制备铜绿假单胞菌生物被膜模型,用银染法观察鉴定。结果银染后普通光学显微镜和扫描电镜观察铜绿假单胞菌生物被膜。结论银染法鉴定铜绿假单胞菌生物被膜简单可靠。
Objective To evaluate the effect of silver stain on the identification of Pseudomonas aeruginosa biofilm. Methods Pseudomonas aeruginosa biofilm model was prepared by in vitro plate method and observed by silver staining. Results After silver staining, the Pseudomonas aeruginosa biofilms were observed by ordinary light microscopy and scanning electron microscopy. Conclusion The silver staining method for the identification of Pseudomonas aeruginosa biofilm is simple and reliable.